Journal: Frontiers in Immunology

Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging

doi: 10.3389/fimmu.2024.1383932

Figure Lengend Snippet: Immunophenotyping panel for multiplexed tissue imaging of cancer.

Article Snippet: CD3 , REA1151 , 50 , 130-120-267 , FITC (APC) , Miltenyi Biotec.

Techniques: Imaging

Journal: Journal of stomatology, oral and maxillofacial surgery

Article Title: Elevated TRPV2 expression in the facial nerve of rats by cold stimulation: Implications for Bell's palsy.

doi: 10.1016/j.jormas.2024.101895

Figure Lengend Snippet: Fig. 4. A: RNA expression of TRPV2. B: Protein expression of TRPV2. The RNA expression values for Day 1, 3 and 7 are 7.255, 2.353, and 1.463. In three groups, RNA expression of TRPV2 is higher on the left side of the facial nerve than on the right side. The protein expression values corresponding to Day 1, 3 and 7 are 0.8791, 1.176, and 1.606. In Day 3 and 7, the protein expression of TRPV2 was elevated but not statistically significant. In Day 1, the protein expression of TRPV2 is marginally lower in the left facial nerve compared to the right nerve. (ns P > 0.05, * P < 0.05, ** P < 0.01).

Article Snippet: TRPV2 Antibody (BOSTER Biological Technology Co., Catalog Number: A02786−3), B-actin Antibody (ABclonal, Catalog Number: AC026), and Secondary Anti-HRP Conjugated AffiniPure Goat Anti-rabbit IgG (BOSTER Biological Technology Co., Catalog Number: BA1054).

Techniques: RNA Expression, Expressing

Journal: Frontiers in Immunology

Article Title: Allograft or Recipient ST2 Deficiency Oppositely Affected Cardiac Allograft Vasculopathy via Differentially Altering Immune Cells Infiltration

doi: 10.3389/fimmu.2021.657803

Figure Lengend Snippet: The effects of recipient or graft ST2 deficiency on CD3 + T cells infiltration in cardiac allograft. (A) The allograft infiltrated CD3 + T cells identified using IHC. (B) Quantification of infiltrated CD3 + T cells (n = 4-6 per group) in the arterial wall of allografts with Image J. HTx: heart transplantation. Data are presented as mean ± SEM. Scale bars are 50 µm. P values were established by 2-way ANOVA.

Article Snippet: Subsequently, sections were incubated with antibodies against CD3 (1:200, #PB9093, Boster Ltd., Wuhan, China), CD20 (1:500, #GB11540, Servicebio, Wuhan, China), and F4/80 (1:200, #70076, Cell Signaling Technology, Inc., Danvers, MA, USA) over night at 4°C to detect the infiltration of T cell, B cell and macrophage into heart, respectively.

Techniques: Transplantation Assay